MRG15, in contrast, is a highly multifunctional gene that affects transcription of other genes involved in multiple pathways, via multiple nucleoprotein complexes, some of which act by chromatin remodeling. However, the antibody used cross-reacts with another protein of 55 kD in Western blot analysis of whole worm lysate, making this interpretation difficult. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Select Format Select format. Kaoru Tominaga for helping with the production of the constructs, Dr.
Lyra. Age: 25.
The injected worms were transferred to a new plate the next morning, and the F 1 progeny were analyzed for various phenotypes.
Holland. Age: 20.
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Overexpression from the cytomegalovirus promoter eventually resulted in cell death, possibly due to competition with hMRG15 in endogenous nucleoprotein complexes. After confirmation by sequencing, the phagemids were linearized with EcoI for T3 reactions of both ykf12 and ykd7; and for T7 reactions with EcoRI for ykf12 and with SpeI for ykd7. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. HeLa, an immortal human cell line derived from a cervical carcinoma, was used in this study.